PIXI
PIXI Immunoassay Platform
Platform
Plate-based immunoassays are the workhorse of protein quantification, but these decades-old methods require tedious manual intervention, have limited dynamic range, require relatively large (50-100 μL) sample volume, and have long assay times.
The PIXI system consists of an assay cartridge and a corresponding module that sits on the deck of an automated liquid handler. Each assay kit includes the necessary reagents and a single 96-test cartridge. PIXI cartridges contain a proprietary nanoporous polymer, which accelerates the molecular interactions required to capture protein targets. After 30 min, users transfer the PIXI cartridge to your own imager to complete the readout. With a 6-log dynamic range and only 1 ul of sample volume required, this automated platform is a game-changer for your laboratory workflow.
Product Workflow
The Definition of Plug and Play
Acheive data in as little as 30min with less than 2 min of hands on time
Setup PIXI module onto liquid handler deck alongside sample plate
Load cartridge into PIXI and press GO to run automated ELISA
Transfer cartridge onto plate imager and read fluorescence output
Analyze ELISA results using integrated PIXI software
key features
Specifications
General
Technical
Performance
General
Detection method
Throughput
Single target
(from sample loading to analyzed data)
30 min for 32 samples (min. throughput)
Technical
Size
Ondeck Module
WDH: 12.8 cm x 33 cm x 7.3 cm, (5 in x 13 in x 3 in)
Waste Module
WDH: 16.5 cm x 38 cm x 20.5 cm, (6.5 in x 15 in x 8 in)
Weight
Ondeck Module
2.8 kg (6.25 lb)
Waste Module
Electrical
100 – 240 VAC, 50/60 Hz, 300 VA
Compatible liquid handlers
Imager compatibility
Performance
Repeatability
Precision
Dynamic log range
Sensitivity
ASSAYS
Off-the-Shelf
Pre-tested and ready-to-use without manipulation. Quality reagents, reproducible and robust results.
Custom
Develop your own assay. PIXI cartridges can be loaded with your unique targets of interests.
Application Note
Measuring anti-PD-1 in immune checkpoint mouse model stimulation using custom PIXI assay
We are continuing to expand our inventory of off-the-shelf assays. Connect with us to learn about assays in development and how our team of experts can help you develop a solution tailored to your specific research needs
Human IgG Immunoassay Kit
Catalog No:
KPX1-PCEA101-001
Quantify human IgG in up to 96 samples. Kit includes a PIXI cartridge precoated with
anti-human IgG F(ab’)₂ fragments as well as all necessary reagents, buffers and consumables
Human IL1-Beta Immunoassay Kit
Catalog No:
KPX1-PCEA201-001
anti-human IL-1 beta antibody as well as all necessary reagents, buffers and consumables.
Customizable Ligand Binding Assay Kit
Catalog No:
KPX1-OP001
Open PIXI cartridge containing 96 wells coated with streptavidin-polymer available for customization with specific capture ligands for target analytes of choice.
Software
The PIXI System Software provides an intuitive user interface to set up your experiment and analyze results.
Now Available!
Accelerate your research with fully automated protein quantification on the PIXI platform
“Accelerate your research with fully automated protein quantification on the PIXI platform”
“I liked the quickness of the assay. It was pretty easy to learn and be able to use quickly.”
“The low sample volume is great!”
“Software is streamlined. This lends to easily training others on using the equipment.”
“This is orders of magnitude better than what I’m used to…1000 ug/ml upper limit of detection, that’s insane. We’re used to 1 to 2 log dynamic range for ELISA so 3.5 log is amazing!”
“It uses so little sample…and it reduced a lot of the pipetting!”